In QB desktop I accept payments & make a deposit to 1 account, then add new lines for subaccounts as a negative number to split the $$ up. Can this be done in QB On-line?

I'd be glad to provide some insights about making a bank deposit in QuickBooks Online, @beadcrazy5-gmail.

When recording a bank deposit in QBO, you can add new lines and direct those various $ amounts to several subaccounts. Take note that each bank deposit creates a separate record in QuickBooks. Make deposits one at a time for each of your deposit slips.

Here's how:

1. Click + New.
2. Choose Bank Deposit.
3. From the Account ▼ dropdown, choose the account you want to put the money into.
4. Click the checkbox for each transaction you want to combine.
5. Make sure the total of the selected transactions matches your deposit slip. Use your deposit slip as a reference.
6. Once done, click Save and close or Save and new.

For more information, you may also check out this article: Record and make bank deposits in QuickBooks Online. It also includes steps on how to review and delete past bank deposits. 

I'm also adding this link to learn how to put payments into the Undeposited Funds account before you combine them into a deposit: Deposit payments into the Undeposited Funds account in QuickBooks Online.

Feel free to comment below if you have further concerns about accepting payments and making a bank deposit. I'll be happy to help you always. Keep safe!