Friday is our payroll day, November 26, its Holiday on the 25th, when should I process and submit paroll?

Thanks for sharing your concern with us, ChinaTrip2020.

In QuickBooks Online (QBO), direct deposit paychecks are required to be submitted to Intuit before 5:00 pm PT, at least 2 banking days prior to the paycheck date. A banking day doesn't include Saturdays and Sundays and days that don't fall on a Federal Reserve Federal holiday, or a state or local holiday.

If your payroll day is Friday, normally, you will process payroll 2 days prior which is Wednesday. However, in your case, you'll want to process the payroll on Tuesday, 24th because of one Holiday. 

To give more ideas about the process of changing your pay schedule using the QBO Standard Payroll, please see this article: Set up and manage payroll schedules.

You might want to keep this article handy in case you have issues with direct deposit: Resolve Direct Deposit Issues.

Feel free to check this article for some links about the payroll tasks in QuickBooks Online: QuickBook Online Payroll Resources.

Be sure to get back to me if you have additional questions managing your payroll in QBO=. It would be my pleasure to help. Have a good one!